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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)

Price and availability

150 768 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71)
  • Suitable for: Dot blot, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1]
    See all RAB29 primary antibodies
  • Description

    Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71)
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C] whole cell lysate.
  • General notes

    This antibody was developed with support from The Michael J. Fox Foundation.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MJF-R24-17-1
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

Images

  • Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
    Dot Blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)

    Dot blot analysis of RAB29 (phospho T71) labeled with ab241062 at 1/1000 dilution.

    Lane 1: RAB29 (phospho T71) peptide.
    Lane 2: RAB29 non-phospho peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
    Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
    All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB3 and LRRK2 [Y1699C], whole cell lysate
    Lane 2 : HEK-293 overexpressing HA-tagged RAB5 and LRRK2 [Y1699C], whole cell lysate
    Lane 3 : HEK-293 overexpressing HA-tagged RAB8 and LRRK2 [Y1699C], whole cell lysate
    Lane 4 : HEK-293 overexpressing HA-tagged RAB10 and LRRK2 [Y1699C], whole cell lysate
    Lane 5 : HEK-293 overexpressing HA-tagged RAB12 and LRRK2 [Y1699C], whole cell lysate
    Lane 6 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate
    Lane 7 : HEK-293 overexpressing HA-tagged RAB35 and LRRK2 [Y1699C], whole cell lysate
    Lane 8 : HEK-293 overexpressing HA-tagged RAB43 and LRRK2 [Y1699C], whole cell lysate

    Lysates/proteins at 0.5 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The blot was scanned with LI-COR Odyssey CLx.

    This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

  • Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
    Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
    All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/500 dilution

    Lane 1 : MLi-2 (100nM for 60min) treated HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
    Lane 2 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
    Lane 3 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 1 µg
    Lane 4 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.3 µg
    Lane 5 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.1 µg

    Secondary
    All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking buffer:  5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins. While MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).

    The blot was scanned with LI-COR Odyssey CLx.

    This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee

  • Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
    Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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