Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (ab241062)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1]
See all RAB29 primary antibodies -
Description
Rabbit monoclonal [MJF-R24-17-1] to RAB29 (phospho T71) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C] whole cell lysate.
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General notes
This antibody was developed with support from The Michael J. Fox Foundation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R24-17-1 -
Isotype
IgG -
Research areas
Images
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Dot blot analysis of RAB29 (phospho T71) labeled with ab241062 at 1/1000 dilution.
Lane 1: RAB29 (phospho T71) peptide.
Lane 2: RAB29 non-phospho peptide.Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB3 and LRRK2 [Y1699C], whole cell lysate
Lane 2 : HEK-293 overexpressing HA-tagged RAB5 and LRRK2 [Y1699C], whole cell lysate
Lane 3 : HEK-293 overexpressing HA-tagged RAB8 and LRRK2 [Y1699C], whole cell lysate
Lane 4 : HEK-293 overexpressing HA-tagged RAB10 and LRRK2 [Y1699C], whole cell lysate
Lane 5 : HEK-293 overexpressing HA-tagged RAB12 and LRRK2 [Y1699C], whole cell lysate
Lane 6 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate
Lane 7 : HEK-293 overexpressing HA-tagged RAB35 and LRRK2 [Y1699C], whole cell lysate
Lane 8 : HEK-293 overexpressing HA-tagged RAB43 and LRRK2 [Y1699C], whole cell lysate
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% BSA/TBST.
The blot was scanned with LI-COR Odyssey CLx.
This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.
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All lanes : Anti-15 Lipoxygenase 1 antibody [EPR22136] (ab242062) at 1/500 dilution
Lane 1 : MLi-2 (100nM for 60min) treated HEK-293 (human epithelial cell line from embryonic kidney) overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
Lane 2 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 3 µg
Lane 3 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 1 µg
Lane 4 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.3 µg
Lane 5 : HEK-293 overexpressing HA-tagged RAB29 and LRRK2 [Y1699C], whole cell lysate at 0.1 µg
Secondary
All lanes : IRDye® 800CW Goat-anti-Rabbit at 1/25000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% BSA/TBST.
The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates the phosphorylation of Rab proteins. While MLi-2 is a potent, selective LARRK2 kinase inhibitor (PMID: 26407721).
The blot was scanned with LI-COR Odyssey CLx.
This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee
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