Anti-DDX50 antibody [EPR5273] (ab109515)
![Anti-DDX50 antibody [EPR5273] (ab109515)](https://www.abcam.com/ps/products/109/ab109515/Images/ab109515-122428-anti-ddx50-antibody-epr5273-western-blot.jpg "Anti-DDX50 antibody [EPR5273] (ab109515)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5273] to DDX50
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-DDX50 antibody [EPR5273]
See all DDX50 primary antibodies -
Description
Rabbit monoclonal [EPR5273] to DDX50 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, K562 and Jurkat whole cell lysate (ab7899); Human thyroid gland adenocarcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5273 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DDX50 antibody [EPR5273] (ab109515)All lanes : Anti-DDX50 antibody [EPR5273] (ab109515) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX50 antibody [EPR5273] (ab109515)](https://www.abcam.com/ps/products/109/ab109515/Images/ab109515-122427-anti-ddx50-antibody-epr5273-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX50 antibody [EPR5273] (ab109515)Immunohistochemical analysis of DDX50 in paraffin embedded Human thyroid gland adenocarcinoma tissue, using ab109515 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-DDX50 antibody [EPR5273] (ab109515)](https://www.abcam.com/ps/products/109/ab109515/Images/ab109515-3-benefits-of-recombinant-antibodies.png)
Anti-DDX50 antibody [EPR5273] (ab109515)
