Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990)

Key features and details

Mouse monoclonal [2F12F3] to Casein Kinase 2 beta
Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human
Isotype: IgG1

Product name

Anti-Casein Kinase 2 beta antibody [2F12F3]
See all Casein Kinase 2 beta primary antibodies
Description

Mouse monoclonal [2F12F3] to Casein Kinase 2 beta
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Casein Kinase 2 beta aa 1 to the C-terminus.
Database link: P67870

Run BLAST with Run BLAST with
Positive control
- Human Casein Kinase 2 beta (AA: FULL(1-215)) recombinant protein, Casein Kinase 2 beta (AA: FULL(1-215))-hIgGFc transfected HEK293 cell lysate, Hela, Jurkat, K562, HepG2, C6, SK-N-SH, NTERA-2, MCF-7, NIH/3T3 cell lysate, MCF-7 cells, HeLa cells, Human cervical cancer tissues, Human colon cancer tissues

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

2F12F3
Isotype

IgG1
Research areas

Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990)

All lanes : Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution

Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : C6 cell lysate
Lane 6 : SK-N-SH cell lysate
Lane 7 : NTERA-2 cell lysate
Lane 8 : MCF-7 cell lysate
Lane 9 : NIH/3T3 cell lysate

Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Casein Kinase 2 beta with ab201990 at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990)

Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Casein Kinase 2 beta with ab201990 at 1/200 dilution.
Flow Cytometry - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990)

Flow cytometric analysis of HeLa cells using ab201990 at 1/200 dilution (green) and negative control (red).
Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990)

Immunofluorescence analysis of MCF-7 cells using ab201990 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990)

All lanes : Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : Casein Kinase 2 beta (AA: FULL(1-215))-hIgGFc transfected HEK293 cell lysate

Predicted band size: 25 kDa
Western blot - Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990)

Anti-Casein Kinase 2 beta antibody [2F12F3] (ab201990) at 1/500 dilution + Human Casein Kinase 2 beta (AA: FULL(1-215)) recombinant protein

Predicted band size: 25 kDa

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