Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CtBP1 antibody [EPR6800] (ab129181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6800] to CtBP1
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CtBP1 antibody [EPR6800]
    See all CtBP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6800] to CtBP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CtBP1 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human colon tissue. WB: SH-SY5Y, Raji, A549 and HeLa whole cell lysate (ab150035). ICC/IF: HeLa cells. Flow Cyt: Raji cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CtBP1 antibody [EPR6800] (ab129181)
    Western blot - Anti-CtBP1 antibody [EPR6800] (ab129181)
    All lanes : Anti-CtBP1 antibody [EPR6800] (ab129181) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (ab129181)
    Immunocytochemistry/ Immunofluorescence - Anti-CtBP1 antibody [EPR6800] (ab129181)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CtBP1 with ab129181 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (ab129181)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CtBP1 antibody [EPR6800] (ab129181)

    ab129181, at 1/100 dilution, staining CtBP1 in formalin-fixed, paraffin-embedded human colon tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-CtBP1 antibody [EPR6800] (ab129181)
    Flow Cytometry - Anti-CtBP1 antibody [EPR6800] (ab129181)

    Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CtBP1 (red) with ab129181 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

  • OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (ab129181)
    OI-RD Scanning - Anti-CtBP1 antibody [EPR6800] (ab129181)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CtBP1 antibody [EPR6800] (ab129181)
    Anti-CtBP1 antibody [EPR6800] (ab129181)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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