Anti-CtBP1 antibody (ab85992)
Key features and details
- Rabbit polyclonal to CtBP1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-CtBP1 antibody
See all CtBP1 primary antibodies -
Description
Rabbit polyclonal to CtBP1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
3 synthetic peptides (Human) conjugated to KLH
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Positive control
- Recombinant protein produced in an induced culture of E.coli.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CtBP1 antibody (ab85992)All lanes : Anti-CtBP1 antibody (ab85992) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
All lanes : anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size: 49 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.
Predicted molecular weight of recombinant CtBP1:61.5kDa (47.5kDa + another 14kDa for the tag). -
Western blot - Anti-CtBP1 antibody (ab85992) This image is courtesy of Anonymous Abreview.All lanes : Anti-CtBP1 antibody (ab85992) at 1/30000 dilution
All lanes : Mouse brain whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 15 seconds
