Anti-BMPR1B antibody [MM0055-3E12] (ab78417)
Key features and details
- Mouse monoclonal [MM0055-3E12] to BMPR1B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-BMPR1B antibody [MM0055-3E12]
See all BMPR1B primary antibodies -
Description
Mouse monoclonal [MM0055-3E12] to BMPR1B -
Host species
Mouse -
Specificity
ab78417 detects BMPR1B. No cross reactivity was found to BMPR1A (ALK3). -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human BMPR1B extracellular domain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline (PBS). -
Clonality
Monoclonal -
Clone number
MM0055-3E12 -
Isotype
IgG2a -
Research areas
Images
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ab78417 at 1/200 dilution staining BMPR1B in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
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All lanes : Anti-BMPR1B antibody [MM0055-3E12] (ab78417) at 1 µg/ml
Lane 1 : Human bone tumor tissue lysate - total protein (ab29359)
Lane 2 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 30 kDa, 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
The band observed at 53 kDa could potentially be a cleaved form of BMPR1B due to presence of a 13 amino acid signal peptide.