Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Horse, Human

Overview

  • Product name

    Anti-Casein Kinase 2 beta antibody [EP1995Y]
    See all Casein Kinase 2 beta primary antibodies

  • Description

    Rabbit monoclonal [EP1995Y] to Casein Kinase 2 beta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Casein Kinase 2 beta aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: K562 cell lysate ICC/IF: HeLa cells IHC-P: Human ovary carcinoma tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP1995Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + K562 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Immunofluorescent staining of Casein Kinase 2 beta in HeLa cells using ab76025 at a 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

    Immunohistochemical analysis of Casein Kinase 2 beta in paraffin embedded human ovary carcinoma tissue using ab76025 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Flow Cytometry - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Overlay histogram showing HeLa cells stained with ab76025 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76025, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Western blot - Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) This image is courtesy of an anonymous Abreview
    Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025) at 1/1000 dilution + MEF whole cell lysate at 20 µg

    Secondary
    Alexa Fluor® 680-conjugated polyclonal goat anti-rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa


    Exposure time: 10 minutes


    Blocked for 30 minutes at 25°C.

    Incubated with the primary antibody for 2 hours at 25°C in blocking buffer.

    See Abreview

  • Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)
    Anti-Casein Kinase 2 beta antibody [EP1995Y] (ab76025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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