Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-2 antibody [EPR17881] (ab182738)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17881] to Caspase-2
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-2 antibody [EPR17881]
    See all Caspase-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17881] to Caspase-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat whole cell lysates treated with 25uM etoposide for 5 hours. Hek-293, SK-BR-3 whole cell lysates. Human thymus tissue lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
    Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)

    Lane 1: Wild-type HAP1 cell lysate (20 µg).
    Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg).
    Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab182738 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab182738 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
    Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
    All lanes : Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution

    Lane 1 : Jurkat whole cell lysates(Human T cell leukemia cells from peripheral blood) treated with 25uM etoposide for 5 hours
    Lane 2 : Untreated Jurkat whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
    Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
    All lanes : Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates
    Lane 2 : SK-BR-3 (Human mammary gland adenocarcinoma) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
    Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
    Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/1000 dilution + Human thymus at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Caspase-2 antibody [EPR17881] (ab182738)
    Anti-Caspase-2 antibody [EPR17881] (ab182738)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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