Anti-Caspase-2 antibody [EPR17881] (ab182738)
![Anti-Caspase-2 antibody [EPR17881] (ab182738)](https://www.abcam.com/ps/products/182/ab182738/Images/ab182738-253280-anti-caspase-2-antibody-epr17881-western-blot.jpg "Anti-Caspase-2 antibody [EPR17881] (ab182738)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17881] to Caspase-2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Caspase-2 antibody [EPR17881]
See all Caspase-2 primary antibodies -
Description
Rabbit monoclonal [EPR17881] to Caspase-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat whole cell lysates treated with 25uM etoposide for 5 hours. Hek-293, SK-BR-3 whole cell lysates. Human thymus tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17881 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)
Lane 1: Wild-type HAP1 cell lysate (20 µg).
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg).
Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.ab182738 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab182738 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)](https://www.abcam.com/ps/products/182/ab182738/Images/ab182738-238945-anti-caspase-2-antibody-epr17881-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)All lanes : Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysates(Human T cell leukemia cells from peripheral blood) treated with 25uM etoposide for 5 hours
Lane 2 : Untreated Jurkat whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)](https://www.abcam.com/ps/products/182/ab182738/Images/ab182738-238946-anti-caspase-2-antibody-epr17881-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)All lanes : Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates
Lane 2 : SK-BR-3 (Human mammary gland adenocarcinoma) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)](https://www.abcam.com/ps/products/182/ab182738/Images/ab182738-238947-anti-caspase-2-antibody-epr17881-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [EPR17881] (ab182738)Anti-Caspase-2 antibody [EPR17881] (ab182738) at 1/1000 dilution + Human thymus at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-Caspase-2 antibody [EPR17881] (ab182738)](https://www.abcam.com/ps/products/182/ab182738/Images/ab182738-6-benefits-of-recombinant-antibodies.png)
Anti-Caspase-2 antibody [EPR17881] (ab182738)
