Recombinant human Caspase-9 protein (Tagged) (ab198061)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 56% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
-
Product name
Recombinant human Caspase-9 protein (Tagged)
See all Caspase-9 proteins and peptides -
Biological activity
Specific Activity: 0.11 pmol/min/μg.
Assay conditions: 50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 0.1% Chaps, 5% glycerol, 10 mM DTT, and 10 μM Caspase 9 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em505 nm.
-
Purity
>= 56 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Sequence
MDEADRRLLRRCRLRLVEELQVDQLWDALLSRELFRPHMIEDIQRAGSGS RRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASFLRTNRQAAKLSK PTLENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESLRGNAD LAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFM VEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAV YGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVAS TSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPG FVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQM PGCFNFLRKKLFFKTSHHHHHH -
Amino acids
1 to 416 -
Tags
His tag C-Terminus -
Additional sequence information
Procaspase- 9 is autocleaved between the prodomain and large protease subunit (p35). The cleavage product, contains both the large (p35) and small (p10 [His-tag]) protease subunits. MWt: 35 & 10 kDa
-
Preparation and Storage
-
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.54% Imidazole
Constituents: 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine), 0.02% Potassium chloride, 0.64% Sodium chloride, 0.61% TrisThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
-
Functional Studies - Recombinant human Caspase-9 protein (Tagged) (ab198061)
Activity assay using ab198122.
-
SDS-PAGE - Recombinant human Caspase-9 protein (Tagged) (ab198061)4-20% SDS-PAGE analysis of 2.5 µg ab198061 with Coomassie staining.
