Antibodies, Proteins, Kits and Reagents for Life Science

257 980 ₸ Product size

200 µl 257 980 ₸

Order now and get it on Wednesday March 17, 2021

Anti-Rab3A antibody [1531CT562.14.57] (ab210943)

Key features and details

Mouse monoclonal [1531CT562.14.57] to Rab3A
Suitable for: WB, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human
Isotype: IgG1

Product name

Anti-Rab3A antibody [1531CT562.14.57]
See all Rab3A primary antibodies
Description

Mouse monoclonal [1531CT562.14.57] to Rab3A
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Rat
ICC/IF	Rat
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human Rab3A.
Database link: P20336
Positive control
- PC-12 cells; mouse, rat and Human brain lysates.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

ab210943 is purified through a protein G column, followed by dialysis against PBS.
Clonality

Monoclonal
Clone number

1531CT562.14.57
Isotype

IgG1
Light chain type

kappa
Research areas

Western blot - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)

All lanes : Anti-Rab3A antibody [1531CT562.14.57] (ab210943) at 1/500 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 24 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized PC-12 cells labeling Rab3A with ab210943 at 1/25 dilution, followed by Dylight^® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Cytoplasmic actin is detected with Dylight^® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Flow Cytometry - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)

Flow cytometric analysis of PC-12 cells labeling Rab3A with ab210943 at 1/25 dilution (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by ab210943 (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight^® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western blot - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)

Anti-Rab3A antibody [1531CT562.14.57] (ab210943) at 1/500 dilution + Human brain lysate at 20 µg

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 24 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com