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Anti-Rab3A antibody [1531CT562.14.57] (ab210943)

Key features and details

  • Mouse monoclonal [1531CT562.14.57] to Rab3A
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Rab3A antibody [1531CT562.14.57]
    See all Rab3A primary antibodies

  • Description

    Mouse monoclonal [1531CT562.14.57] to Rab3A

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC/IF
    Rat
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Rab3A.
    Database link: P20336

  • Positive control

    • PC-12 cells; mouse, rat and Human brain lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    ab210943 is purified through a protein G column, followed by dialysis against PBS.

  • Clonality

    Monoclonal

  • Clone number

    1531CT562.14.57

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)
    Western blot - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)
    All lanes : Anti-Rab3A antibody [1531CT562.14.57] (ab210943) at 1/500 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 24 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized PC-12 cells labeling Rab3A with ab210943 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

  • Flow Cytometry - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)
    Flow Cytometry - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)

    Flow cytometric analysis of PC-12 cells labeling Rab3A with ab210943 at 1/25 dilution (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by ab210943 (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG (1μg/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

  • Western blot - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)
    Western blot - Anti-Rab3A antibody [1531CT562.14.57] (ab210943)
    Anti-Rab3A antibody [1531CT562.14.57] (ab210943) at 1/500 dilution + Human brain lysate at 20 µg

    Secondary
    Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 24 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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