Anti-beta Catenin (phospho Y489) antibody (ab119801)
Key features and details
- Rabbit polyclonal to beta Catenin (phospho Y489)
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-beta Catenin (phospho Y489) antibody
See all beta Catenin primary antibodies -
Description
Rabbit polyclonal to beta Catenin (phospho Y489) -
Host species
Rabbit -
Specificity
Immunogen has high homology to the conserved site in gamma-Catenin (Y480). -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
-
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding tyrosine 489 of Human beta Catenin.
-
Positive control
- A431 cells and cell lysates treated with pervanadate.
-
General notes
Do not aliquot.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab119801 was cross-adsorbed to phospho-beta Catenin (phospho Y654) peptide before affinity purification using phospho-beta Catenin (Y489) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-beta Catenin (phospho Y489) antibody (ab119801)Lanes 1-2 : Anti-gamma Catenin at 1/1000 dilution
Lanes 3-4 : Anti-beta Catenin (phospho Y489) antibody (ab119801) at 1/1000 dilution
Lanes 5-6 : Anti-beta Catenin at 1/1000 dilution
Lanes 1 & 3 & 5 : A431 cell lysate, stimulated with
pervanadate (1 mM) for 30 min
Lane 2 : A431 cell lysate, stimulated with
pervanadate (1 mM) for 30 min then
treated with akaline phosphatase
Lanes 4 & 6 : A431 cell lysate, stimulated with
pervanadate (1 mM) for 30 min then treated with akaline phosphatase
Predicted band size: 85 kDa
-
Immunocytochemistry - Anti-beta Catenin (phospho Y489) antibody (ab119801)ab119801 at 1/250 dilution staining beta Catenin in A431 cells treated with pervanadate by Immunocytochemistry followed by detection with secondary antibody conjugated to Cy3.
