Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human RHOC ELISA Kit (ab243680)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 140 pg/ml
  • Range: 0.78 ng/ml - 50 ng/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human RHOC ELISA Kit
    See all RHOC kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    cell extract 3 5.3%
    Inter-assay
    Sample n Mean SD CV%
    cell extract 7 7.3%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    140 pg/ml

  • Range

    0.78 ng/ml - 50 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 97 96% - 98%
    Tissue Extracts 97 96% - 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human RHOC ELISA Kit (ab243680) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RHOC protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human RHOC with 140 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human RHOC Capture Antibody 1 x 600µl
    10X Human RHOC Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human RHOC Lyophilized recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers.

  • Sequence similarities

    Belongs to the small GTPase superfamily. Rho family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Alternative names

    • Aplysia RAS-related homolog 9
    • ARH 9
    • ARH9
    • ARHC
    • H9
    • MGC1448
    • MGC61427
    • Oncogene RHO H9
    • Ras homolog gene family member C
    • RAS related homolog 9
    • RHO C
    • Rho cDNA clone 9
    • Rho related GTP binding protein RhoC
    • Rho-related GTP-binding protein RhoC
    • rhoC
    • RhoC GTPase
    • RHOC_HUMAN
    • RHOH 9
    • RHOH9
    • Small GTP binding protein RhoC
    see all

  • Database links

    Images

    • Other - Human RHOC ELISA Kit (ab243680)
      Other - Human RHOC ELISA Kit (ab243680)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human RHOC standard curve in 1X Cell Extraction Buffer PTR.
      Example of human RHOC standard curve in 1X Cell Extraction Buffer PTR.

      The RHOC standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native RHOC in MCF7, HUVEC, SH-SY5Y, mouse NIH/3T3, and rat liver extracts.
      Interpolated concentrations of native RHOC in MCF7, HUVEC, SH-SY5Y, mouse NIH/3T3, and rat liver extracts.

      Interpolated concentrations of native RHOC in MCF7, HUVEC, SH-SY5Y, mouse NIH/3T3, and rat liver extracts based on a 300 μg/mL extract load for MCF7 and HUVEC samples, 800 μg/mL for SH-SY5Y sample, 400 μg/mL extract load for NIH/3T3 and rat liver samples. The concentrations of RHOC were measured in duplicate and interpolated from the RHOC standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Sandwich ELISA - Human RHOC ELISA Kit (ab243680)
      Sandwich ELISA - Human RHOC ELISA Kit (ab243680)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Human RHOC ELISA Kit (ab243680)

    © 2021 Astana Biomed Group LLP. All rights reserved.