Anti-Rab3A antibody (ab80192)
Key features and details
- Rabbit polyclonal to Rab3A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Rab3A antibody
See all Rab3A primary antibodies -
Description
Rabbit polyclonal to Rab3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Pig, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human Rab3A aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90256) -
Positive control
- This antibody gave a positive signal in the following lysates: Human Brain tissue; Human Brain membrane extract; Rat Brain Tissue; Mouse Brain Tissue; PC12 Whole Cell.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab80192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. -
Tissue specificity
Specifically expressed in brain. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 282029 Cow
- Entrez Gene: 5864 Human
- Entrez Gene: 19339 Mouse
- Entrez Gene: 25531 Rat
- Omim: 179490 Human
- SwissProt: P11023 Cow
- SwissProt: P20336 Human
- SwissProt: P63011 Mouse
see all -
Alternative names
- Rab 3A antibody
- RAB 3A member RAS oncogene family antibody
- Rab3a antibody
see all
Images
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Western blot - Anti-Rab3A antibody (ab80192)All lanes : Anti-Rab3A antibody (ab80192) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Ras-related protein Rab-3A contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight (26 kDa) than predicted (24 kDa). -
Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab80192)ICC/IF image of ab80192 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80192, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Rab3A antibody (ab80192)All lanes : Anti-Rab3A antibody (ab80192) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
Western blot - Anti-Rab3A antibody (ab80192)
Protocols
Datasheets and documents
References (0)
ab80192 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Rab3A antibody (ab80192)All lanes : Anti-Rab3A antibody (ab80192) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Ras-related protein Rab-3A contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight (26 kDa) than predicted (24 kDa). -
Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab80192)ICC/IF image of ab80192 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80192, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Rab3A antibody (ab80192)All lanes : Anti-Rab3A antibody (ab80192) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
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Western blot - Anti-Rab3A antibody (ab80192)
