Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

200 µl 291 484 ₸

Order now and get it on Wednesday March 03, 2021

Key features and details

Rabbit polyclonal to Rab3A
Suitable for: WB, ICC/IF
Reacts with: Mouse, Rat, Human
Isotype: IgG

Product name

Anti-Rab3A antibody
See all Rab3A primary antibodies
Description

Rabbit polyclonal to Rab3A
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Pig, Zebrafish
Immunogen

Synthetic peptide corresponding to Human Rab3A aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab90256)
Positive control
- This antibody gave a positive signal in the following lysates: Human Brain tissue; Human Brain membrane extract; Rat Brain Tissue; Mouse Brain Tissue; PC12 Whole Cell.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
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Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Recombinant Protein
- Recombinant Human Rab3A protein (ab109964)

Our Abpromise guarantee covers the use of ab80192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).
ICC/IF		Use a concentration of 1 µg/ml.

Function

Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.
Tissue specificity

Specifically expressed in brain.
Sequence similarities

Belongs to the small GTPase superfamily. Rab family.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P20336 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 282029 Cow
- Entrez Gene: 5864 Human
- Entrez Gene: 19339 Mouse
- Entrez Gene: 25531 Rat
- Omim: 179490 Human
- SwissProt: P11023 Cow
- SwissProt: P20336 Human
- SwissProt: P63011 Mouse
- SwissProt: Q06AU3 Pig
- SwissProt: P63012 Rat
- Unigene: 27744 Human
- Unigene: 5083 Mouse
- Unigene: 44409 Rat
see all
Alternative names
- Rab 3A antibody
- RAB 3A member RAS oncogene family antibody
- Rab3a antibody
- RAB3A member RAS oncogene family antibody
- RAB3A_HUMAN antibody
- RAS associated protein RAB 3A antibody
- RAS associated protein RAB3A antibody
- Ras related protein Rab 3A antibody
- Ras related protein Rab3A antibody
- Ras-related protein Rab-3A antibody
see all

Western blot - Anti-Rab3A antibody (ab80192)

All lanes : Anti-Rab3A antibody (ab80192) at 1 µg/ml

Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 24 kDa
Observed band size: 26 kDa
why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 30 seconds

Ras-related protein Rab-3A contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight (26 kDa) than predicted (24 kDa).
Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab80192)

ICC/IF image of ab80192 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80192, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Rab3A antibody (ab80192)

All lanes : Anti-Rab3A antibody (ab80192) at 1 µg/ml

Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?

Exposure time: 2 minutes
Western blot - Anti-Rab3A antibody (ab80192)

Western blot protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheet

Publishing research using ab80192? Please let us know so that we can cite the reference in this datasheet.

ab80192 has not yet been referenced specifically in any publications.

Western blot - Anti-Rab3A antibody (ab80192)

All lanes : Anti-Rab3A antibody (ab80192) at 1 µg/ml

Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 24 kDa
Observed band size: 26 kDa
why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 30 seconds

Ras-related protein Rab-3A contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight (26 kDa) than predicted (24 kDa).
Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab80192)

ICC/IF image of ab80192 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80192, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Rab3A antibody (ab80192)

All lanes : Anti-Rab3A antibody (ab80192) at 1 µg/ml

Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 24 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?

Exposure time: 2 minutes
Western blot - Anti-Rab3A antibody (ab80192)