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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-Rho antibody [Y486] (ab32046)

Price and availability

321 638 ₸

Availability

Order now and get it on Tuesday March 16, 2021

Anti-Rho antibody [Y486] (ab32046)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y486] to Rho
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-Rho antibody [Y486]
    See all Rho primary antibodies
  • Description

    Rabbit monoclonal [Y486] to Rho
  • Host species

    Rabbit
  • Specificity

    The antibody is predicted to detect RhoA, RhoB and RhoC based on sequence analysis.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Rho aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • PC12 cell lysate and hepatocellular carcinoma.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    Y486
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

Images

  • Western blot - Anti-Rho antibody [Y486] (ab32046)
    Western blot - Anti-Rho antibody [Y486] (ab32046)
    Anti-Rho antibody [Y486] (ab32046) at 1/2000 dilution + PC12 cell lysate

    Predicted band size: 21 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [Y486] (ab32046)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [Y486] (ab32046)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab32046 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Rho antibody [Y486] (ab32046)
    Anti-Rho antibody [Y486] (ab32046)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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