Recombinant Human RALA protein (ab102555)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: WB, MS, SDS-PAGE
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Product name
Recombinant Human RALA protein
See all RALA proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab102555 was purified using conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMGSHMAANKPKGQNSLALHKVIMVGSGGVG KSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYA AIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRVKEDENVPFLLVG NKSDLEDKRQVSVEEAKNRAEQWNVNYVETSAKTRANVDKVFFDLMREIR ARKMEDSKEKNGKKKRKSLAKRIRERC -
Predicted molecular weight
26 kDa including tags -
Amino acids
1 to 203 -
Tags
His tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.00174% PMSF, 0.0308% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
Images
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SDS-PAGE - Recombinant Human RALA protein (ab102555)15% SDS-PAGE analysis of 3µg of ab102555.
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Western blot - Recombinant Human RALA protein (ab102555)Anti-RALA antibody (ab96759) at 1/3000 dilution +Recombinant Human RALA protein (ab102555) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 4 minutesab996759 recognizes the full length tagged recombinant protein ab102555 which has an expected molecular weight of 30 kDa.
