Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rab11A antibody [EPR7587(B)] (ab128913)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7587(B)] to Rab11A
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Rab11A antibody [EPR7587(B)]
    See all Rab11A primary antibodies

  • Description

    Rabbit monoclonal [EPR7587(B)] to Rab11A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Rab11A aa 150 to the C-terminus.
    Database link: P62491

  • Positive control

    • WB: PC12, HeLa and A549 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (unpurified)

    Lane 1 : PC12 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified)

    Lane 1 : Rat brain lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    All lanes : Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified)

    Lane 1 : Mouse brain cell lysate
    Lane 2 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa

  • Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Western blot - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Anti-Rab11A antibody [EPR7587(B)] (ab128913) at 1/10000 dilution (purified) + A549 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Immunoprecipitation - Anti-Rab11A antibody [EPR7587(B)] (ab128913)

    ab128913 (purified) at 1/20 immunoprecipitating Rab11A in A549 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/10,000) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Anti-Rab11A antibody [EPR7587(B)] (ab128913)
    Anti-Rab11A antibody [EPR7587(B)] (ab128913)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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