Anti-Rab11A antibody (ab65200)
Key features and details
- Rabbit polyclonal to Rab11A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rab11A antibody
See all Rab11A primary antibodies -
Description
Rabbit polyclonal to Rab11A -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Sheep, Chicken, Cow, Rhesus monkey
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Rab11.
Read Abcam's proprietary immunogen policy (Peptide available as ab66706.) -
Positive control
- Recombinant Human Rab11A protein (ab117177) can be used as a positive control in WB. This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, MCF7, SHSY-5Y
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab65200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Regulates endocytic recycling. May exert its functions by interacting with multiple effector proteins in different complexes. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis (By similarity). Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. -
Sequence similarities
Belongs to the small GTPase superfamily. Rab family. -
Cellular localization
Cell membrane. Recycling endosome membrane. Cleavage furrow. Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis. - Information by UniProt
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Database links
- Entrez Gene: 415544 Chicken
- Entrez Gene: 534708 Cow
- Entrez Gene: 8766 Human
- Entrez Gene: 53869 Mouse
- Omim: 605570 Human
- SwissProt: Q5ZJN2 Chicken
- SwissProt: Q2TA29 Cow
- SwissProt: P62491 Human
see all -
Alternative names
- MGC1490 antibody
- Rab 11 antibody
- Rab 11A antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Rab11A antibody (ab65200)ICC/IF image of ab65200 stained MCF-7 cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65200 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol (5 min) fixed MCF7 cells at 5µg/ml.
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Western blot - Anti-Rab11A antibody (ab65200)All lanes : Anti-Rab11A antibody (ab65200) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Additional bands at: 26 kDa (possible post-translational modification), 45 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
We believe that the additional bands observed at 45 and 72 kDa may be a result of disulphide-linked forms of the Rab11 protein
Protocols
References (20)
ab65200 has been referenced in 20 publications.
- Actis Dato V et al. LRP1-Mediated AggLDL Endocytosis Promotes Cholesteryl Ester Accumulation and Impairs Insulin Response in HL-1 Cells. Cells 9:N/A (2020). PubMed: 31936892
- Li HY et al. Matrix sieving-enforced retrograde transcytosis regulates tissue accumulation of C-reactive protein. Cardiovasc Res 115:440-452 (2019). PubMed: 29992240
- Genoyer E & López CB Defective Viral Genomes Alter How Sendai Virus Interacts with Cellular Trafficking Machinery, Leading to Heterogeneity in the Production of Viral Particles among Infected Cells. J Virol 93:N/A (2019). PubMed: 30463965
- Faris R et al. Chlamydia trachomatis CT229 Subverts Rab GTPase-Dependent CCV Trafficking Pathways to Promote Chlamydial Infection. Cell Rep 26:3380-3390.e5 (2019). PubMed: 30893609
- Song J & Wang C Transcriptomic and proteomic analyses of genetic factors influencing adductor muscle coloration in QN Orange scallops. BMC Genomics 20:363 (2019). PubMed: 31072381
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Rab11A antibody (ab65200)ICC/IF image of ab65200 stained MCF-7 cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65200 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol (5 min) fixed MCF7 cells at 5µg/ml.
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Western blot - Anti-Rab11A antibody (ab65200)All lanes : Anti-Rab11A antibody (ab65200) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDa
Additional bands at: 26 kDa (possible post-translational modification), 45 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
We believe that the additional bands observed at 45 and 72 kDa may be a result of disulphide-linked forms of the Rab11 protein
