Anti-FER (phospho Y402) antibody (ab79573)
Key features and details
- Rabbit polyclonal to FER (phospho Y402)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FER (phospho Y402) antibody
See all FER primary antibodies -
Description
Rabbit polyclonal to FER (phospho Y402) -
Host species
Rabbit -
Specificity
ab79573 detects endogenous levels of FER only when phosphorylated at tyrosine 402. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
-
Immunogen
Synthetic phosphopeptide derived from human FER around the phosphorylation site of tyrosine 402 (V-N-YP-E-E)
-
Positive control
- Human brain tissue; Jurkat cell extracts starved for 24 hours.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FER (phospho Y402) antibody (ab79573)ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
-
Western blot - Anti-FER (phospho Y402) antibody (ab79573)All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution
Lane 1 : Jurkat cell extracts, starved (24 hours)
Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
