Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rho antibody [EP487Y] (ab40673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP487Y] to Rho
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Rho antibody [EP487Y]
    See all Rho primary antibodies

  • Description

    Rabbit monoclonal [EP487Y] to Rho

  • Host species

    Rabbit

  • Specificity

    This antibody is specific for Rho. It is also expected to detect RhoA, RhoB and RhoC.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Rho (N terminal). The exact sequence is proprietary.

  • Positive control

    • HL60 cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP487Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    All lanes : Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified)

    Lane 1 : A431 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 22 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [EP487Y] (ab40673)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho antibody [EP487Y] (ab40673)

    Immunohistochemical staining of paraffin embedded human thyroid carcinoma with purified ab40673 at a working dilution of 1 in 250. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-Rho antibody [EP487Y] (ab40673)
    Immunocytochemistry/ Immunofluorescence - Anti-Rho antibody [EP487Y] (ab40673)

    Immunofluorescence staining of MCF7 cells with purified ab40673 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used as primary, followed by an Alexa Fluor® 488 goat anti-rabbit secondary (ab150077) at a dilution of 1/500.

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + Mouse kidney tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + C6 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Anti-Rho antibody [EP487Y] (ab40673) at 1/5000 dilution (purified) + HL-60 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Western blot - Anti-Rho antibody [EP487Y] (ab40673)
    Anti-Rho antibody [EP487Y] (ab40673) at 1/1000 dilution (purified) + MDA-MB-435 at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L)

    Predicted band size: 21 kDa
    Observed band size: 22 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-Rho antibody [EP487Y] (ab40673)
    Anti-Rho antibody [EP487Y] (ab40673)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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