Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3816] to P2Y6
Suitable for: WB, IP, IHC-P, ICC
Reacts with: Human

Product name

Anti-P2Y6 antibody [EPR3816]
See all P2Y6 primary antibodies
Description

Rabbit monoclonal [EPR3816] to P2Y6
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to residues in Human P2Y6 (UniProt ID: Q15077).
Positive control
- A549, HACAT, HepG2, and Human spleen lysates; Human kidney tissue, Human liver tissue. IP: HaCaT whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3816
Isotype

IgG
Research areas

Western blot - Anti-P2Y6 antibody [EPR3816] (ab92504)

All lanes : Anti-P2Y6 antibody [EPR3816] (ab92504) at 1/20000 dilution

Lane 1 : A549 cell lysate
Lane 2 : HACAT cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa
Immunocytochemistry - Anti-P2Y6 antibody [EPR3816] (ab92504)

Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling P2Y6 with purified ab92504 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.20 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y6 antibody [EPR3816] (ab92504)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue (left-hand image) and Human liver tissue (right-hand image) using ab92504 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-P2Y6 antibody [EPR3816] (ab92504)

Purified ab92504 at 1/50 dilution (2µg) immunoprecipitating P2Y6 in HaCaT whole cell lysate.
Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10µg
Lane 2 (+): ab92504 + HaCaT whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92504 in HaCaT whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 42 kDa
Anti-P2Y6 antibody [EPR3816] (ab92504)

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