Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-P2Y6 antibody [EPR3816] (ab92504)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3816] to P2Y6
  • Suitable for: WB, IP, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-P2Y6 antibody [EPR3816]
    See all P2Y6 primary antibodies

  • Description

    Rabbit monoclonal [EPR3816] to P2Y6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human P2Y6 (UniProt ID: Q15077).

  • Positive control

    • A549, HACAT, HepG2, and Human spleen lysates; Human kidney tissue, Human liver tissue. IP: HaCaT whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-P2Y6 antibody [EPR3816] (ab92504)
    Western blot - Anti-P2Y6 antibody [EPR3816] (ab92504)
    All lanes : Anti-P2Y6 antibody [EPR3816] (ab92504) at 1/20000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : HACAT cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 36 kDa

  • Immunocytochemistry - Anti-P2Y6 antibody [EPR3816] (ab92504)
    Immunocytochemistry - Anti-P2Y6 antibody [EPR3816] (ab92504)
    Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling P2Y6 with purified ab92504 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.20 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y6 antibody [EPR3816] (ab92504)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y6 antibody [EPR3816] (ab92504)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue (left-hand image) and Human liver tissue (right-hand image) using ab92504 at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-P2Y6 antibody [EPR3816] (ab92504)
    Immunoprecipitation - Anti-P2Y6 antibody [EPR3816] (ab92504)

    Purified ab92504 at 1/50 dilution (2µg) immunoprecipitating P2Y6 in HaCaT whole cell lysate.
    Lane 1 (input): HaCaT (Human skin keratinocyte) whole cell lysate 10µg
    Lane 2 (+): ab92504 + HaCaT whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92504 in HaCaT whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 42 kDa

  • Anti-P2Y6 antibody [EPR3816] (ab92504)
    Anti-P2Y6 antibody [EPR3816] (ab92504)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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