Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19085] to Phospholipase C beta 1/PLCB1 - BSA and Azide free
  • Suitable for: WB, IHC-Fr, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free
    See all Phospholipase C beta 1/PLCB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19085] to Phospholipase C beta 1/PLCB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HepG2 cell lysates; Mouse heart tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue; Mouse hippocampus tissue.

  • General notes

    ab240260 is the carrier-free version of ab182359 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab240260 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Phospholipase C beta 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Western blot - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PLCB1 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Mouse heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 138 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab182359).

    Lanes 1-4: Merged signal (red and green). Green - ab182359 observed at 150 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab182359 Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] was shown to specifically react with Phospholipase C beta 1/PLCB1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266033 (knockout cell lysate ab257589) was used.  Wild-type and Phospholipase C beta 1/PLCB1 knockout samples were subjected to SDS-PAGE.  ab182359 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on normal Human cerebrum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on mouse hippocampus is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on rat cerebrum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Immunohistochemistry (Frozen sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

    Immunohistochemical analysis of paraffin-embedded frozen, 0.2% Triton X-100 permeabilized Mouse cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).

    The result showed cytoplasmic staining on mouse cerebrum. 

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human colon. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

    Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human gastric cancer. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat kidney. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182359).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)
    Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] - BSA and Azide free (ab240260)

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