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Anti-PLCL2 antibody - C-terminal (ab199852)

Key features and details

  • Rabbit polyclonal to PLCL2 - C-terminal
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PLCL2 antibody - C-terminal

  • Description

    Rabbit polyclonal to PLCL2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PLCL2 aa 1050 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_001137854.1).
    Database link: Q9UPR0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa, 293T, and Jurkat whole cell lysates.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PLCL2 antibody - C-terminal (ab199852)
    Western blot - Anti-PLCL2 antibody - C-terminal (ab199852)
    All lanes : Anti-PLCL2 antibody - C-terminal (ab199852) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 126 kDa


    Exposure time: 3 minutes


    Lysates prepared wtih NETN lysis buffer.

  • Immunoprecipitation - Anti-PLCL2 antibody - C-terminal (ab199852)
    Immunoprecipitation - Anti-PLCL2 antibody - C-terminal (ab199852)

    Detection of PLCL2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab199852 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

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