Human VCAM-1 ELISA Set (without plates) (ab47355)
Overview
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Product name
Human VCAM-1 ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
1.56 ng/ml - 50 ng/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s VCAM-1 Human in vitro ELISA Set is designed for the quantitative measurement of VCAM-1 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human VCAM-1 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The VCAM-1 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human VCAM1
Tested applications
Suitable for: Sandwich ELISAmore detailsPlatform
ReagentsProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-VCAM-1 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials VCAM-1 Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml VCAM1 standard 5 vials 10 vials 15 vials -
Research areas
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Relevance
VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells. -
Cellular localization
Cell Membrane; single-pass type I membrane protein -
Alternative names
- CD106
- CD106 antigen
- INCAM 100
see all -
Database links
- Entrez Gene: 7412 Human
- SwissProt: P19320 Human
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests Detection biotinylated anti-VCAM-1 antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Streptavidin-HRP 1 vial 2 vials 3 vials VCAM-1 Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml VCAM1 standard 5 vials 10 vials 15 vials -
Research areas
-
Relevance
VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells. -
Cellular localization
Cell Membrane; single-pass type I membrane protein -
Alternative names
- CD106
- CD106 antigen
- INCAM 100
see all -
Database links
- Entrez Gene: 7412 Human
- SwissProt: P19320 Human
Images
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Human VCAM-1 ELISA Set (ab47355)VCAM-1 Standard curve ranging from 1.56 to 50 ng/mL