Anti-MCEE antibody [OTI1B5] (ab236397)
Key features and details
- Mouse monoclonal [OTI1B5] to MCEE
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-MCEE antibody [OTI1B5] -
Description
Mouse monoclonal [OTI1B5] to MCEE -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human MCEE. Produced in E.coli (NP_115990).
Database link: Q96PE7 -
Positive control
- WB: pCMV6-ENTRY MCEE cDNA transfected HEK-293T cell lysate. IHC-P: Human colon adenocarcinoma, pancreas carcinoma, endometrium adenocarcinoma, prostate, prostate adenocarcinoma and bladder tissue.
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General notes
Clone OTI1B5 (formerly 1B5).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI1B5 -
Isotype
IgG2b
Images
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All lanes : Anti-MCEE antibody [OTI1B5] (ab236397) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY MCEE cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 19 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (ab236397)
Paraffin-embedded human bladder tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (ab236397)
Paraffin-embedded human prostate carcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (ab236397)
Paraffin-embedded human prostate tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (ab236397)
Paraffin-embedded human endometrium adenocarcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (ab236397)
Paraffin-embedded human pancreas carcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCEE antibody [OTI1B5] (ab236397)
Paraffin-embedded human colon adenocarcinoma tissue stained for MCEE using ab236397 at 1/150 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using Tris-EDTA, pH 8.0.