Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Friday March 05, 2021

Key features and details

Mouse monoclonal [14B4] to Rad51
Suitable for: IHC-P, WB
Reacts with: Human
Isotype: IgG2b

Product name

Anti-Rad51 antibody [14B4]
See all Rad51 primary antibodies
Description

Mouse monoclonal [14B4] to Rad51
Host species

Mouse
Specificity

Specific for human Rad51.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human Rad51 aa 1-338.
Database link: Q06609
Positive control
- WB: RAD51 shRNA transfected (+) 293T whole cell extracts, Jurkat, Raji and NCI-H929 whole cell lysate. IHC-P: BT483 xenoograft tissue, human colon esophagus tissue.
General notes

This product was changed from ascites to tissue culture supernatant on 17^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.40
Constituent: 100% PBS
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

14B4
Myeloma

NS1
Isotype

IgG2b
Light chain type

kappa
Research areas

Western blot - Anti-Rad51 antibody [14B4] (ab213)

All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution

Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : NCI-H929 whole cell lysate

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody at 1/10000 dilution

Developed using the ECL technique.

Predicted band size: 37 kDa

10% SDS-PAGE

Running condition: 80V, 15min; 140V, 40 min

Transfer condition: Semi-dry, 18 V, 60 min (Nitrocellulose membrane)

Blocking condition: 5% non-fat milk in TBST, RT, 60 min.

Primary antibody incubation: 4°C overnight.

Washing condition: 5 ml TBST, 4 x 5 min.

Chemiluminescent HRP Substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H₂O₂, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
Western blot - Anti-Rad51 antibody [14B4] (ab213)

All lanes : Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution

Lane 1 : Non-transfected 293T (-) whole cell extracts
Lane 2 : RAD51 shRNA transfected (+) 293T whole cell extracts

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : HRP-conjugated anti-mouse IgG antibody

Developed using the ECL technique.

Predicted band size: 37 kDa

10% SDS-PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Rad51 with ab213 at 1/100 dilution at 4°C overnight. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Endogenous peroxidase blocking: 3% H₂O₂, RT, 30minutes. Blocking: 1.5% goat serum (dilute goat serum by 1xPBS), RT, 30 min. Secondary antibody: ABC HRP Kit (Mouse IgG), 1:200, RT, 30min. Washing: PBS, 2 x 5 mins. Chromogen system: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213)

Immunohistochemical analysis of paraffin-embedded BT483 xenoograft tissue labeling RAD51 with ab213 at 1/200 dilution. Nuclear staining is observed. Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min.
Western blot - Anti-Rad51 antibody [14B4] (ab213) Image courtesy of an anonymous Abreview.

Anti-Rad51 antibody [14B4] (ab213) at 1/500 dilution + lysate prepared from murine NIH3T3 cells at 20 µg

Secondary
HRP conjugated anti-mouse IgG at 1/5000 dilution

Developed using the ECL technique.

Predicted band size: 37 kDa
Observed band size: 42,45 kDa
why is the actual band size different from the predicted?

Exposure time: 5 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [14B4] (ab213) This image is courtesy of an anonymous Abreview

ab213 staining Rad51 in Human Lovo xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in TBST) for 1 hour. An undiluted HRP-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.
See Abreview

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