Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, IHC-Fr
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free
    See all VDAC1 / Porin primary antibodies

  • Description

    Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2, Jurkat, HEK-293, HAP1 and HeLa cell lysates; Mouse and rat kidney lysate; Rat cerebellum whole tissue lysate IHC-P: Human liver, heart, kidney, ovarian carcinoma, thyroid gland carcinoma, skeletal muscle and cervical carcinoma tissues; Rat kidney tissue; Mouse cardiac muscle tissue; ICC/IF: HeLa and Jurkat cells; IHC-Fr: Mouse cardiac and skeletal muscle tissues.

  • General notes

    ab240128 is the carrier-free version of ab154856. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240128 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Western blot - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    All lanes : Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/1000 dilution

    Lane 1 : Wild-type Hap1 cell lysate
    Lane 2 : VDCA1 knockout Hap1 cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : VDAC1 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab154856).

    Lanes 1-4: Merged signal (red and green). Green - ab154856 observed at 32 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab154856 was shown to react with VDAC1 / Porin in wild-type Hap1 and HEK-293T. Loss of signal was observed when HeLa knockout sample ab263839 and Hap1 knockout sample were used. Wild-type and VDAC1 / Porin knockout samples were subjected to SDS-PAGE. ab154856 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)  and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Western blot - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    All lanes : Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : VDAC1 knockout HAP1 whole cell lysate
    Lane 3 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab154856 observed at 31 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab154856 was shown to specifically react with in wild-type HAP1 cells as signal was lost in VDAC1 knockout cells. Wild-type and VDAC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab154856 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

  • Immunohistochemistry (Frozen sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Frozen sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    Immunohistochemistry (Frozen sections) analysis of mouse skeletal muscle tissue sections labeling VDAC1 / Porin with Purified ab154856 at 1/50 (0.7 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    ab154856 staining VDAC1 / Porin showing cytoplasmic staining in Jurkat cells (Human T cell leukemia T lymphocyte) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol, Samples were incubated with primary antibody (1/1000) for 1 hour at 21°C. ab150077, an Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG (1:1000) was used as the secondary antibody. DAPI (1/200) was used as a counter stain. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemical staining of paraffin embedded rat kidney with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    ab154856 staining VDAC1 / Porin showing cytoplasmic staining in HeLa cells (Human cervix adenocarcinoma epithelial cells) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol, Samples were incubated with primary antibody (1/1000) for 1 hour at 21°C. ab150077, an Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG (1:1000) was used as the secondary antibody. DAPI (1/200) was used as a counter stain. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VDAC1 with unpurified ab154856 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    Immunohistochemical analysis of paraffin-embedded human heart tissue labeling VDAC1 with unpurified ab154856 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    Immunohistochemical analysis of paraffin embedded human normal kidney tissue using unpurified ab154856 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue using unpurified ab154856 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    Immunohistochemical analysis of paraffin embedded human thyroid gland carcinoma tissue using unpurified ab154856 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

    Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using unpurified ab154856 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab154856).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)
    Anti-VDAC1 / Porin antibody [EPR10852(B)] - BSA and Azide free (ab240128)

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