HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - Mitochondrial Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control
See all VDAC1 / Porin primary antibodies -
Description
HRP Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - Mitochondrial Loading Control -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, HeLa, HEK-293 HAP1 and Jurkat, whole cell and Human Kidney, Mouse Kidney and Rat Kidney tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10852(B) -
Isotype
IgG -
Research areas
Images
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All lanes : HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 :HeLa whole cell lysate (ab150035)
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : Kidney (Rat) Tissue Lysate
Lane 6 : Kidney (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185063 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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Clone EPR10852(B) has been knock-out validated in its unconjugated form. Please refer to ab154856 datasheet for experimental details.
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