Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

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HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
HRP Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - Mitochondrial Loading Control
Suitable for: WB
Reacts with: Mouse, Rat, Human
Conjugation: HRP

Product name

HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control
See all VDAC1 / Porin primary antibodies
Description

HRP Rabbit monoclonal [EPR10852(B)] to VDAC1 / Porin - Mitochondrial Loading Control
Host species

Rabbit
Conjugation

HRP
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, HeLa, HEK-293 HAP1 and Jurkat, whole cell and Human Kidney, Mouse Kidney and Rat Kidney tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10852(B)
Isotype

IgG
Research areas

Western blot - HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)

All lanes : HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063) at 1/5000 dilution

Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa whole cell lysate (ab150035)
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : Kidney (Rat) Tissue Lysate
Lane 6 : Kidney (Human) Tissue Lysate - adult normal tissue

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 31 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?

Exposure time: 4 minutes

This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab185063 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Western blot - HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)

Clone EPR10852(B) has been knock-out validated in its unconjugated form. Please refer to ab154856 datasheet for experimental details.
HRP Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab185063)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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