Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)
Key features and details
- Rabbit polyclonal to VDAC1 / Porin - Mitochondrial Loading Control
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Dog, Human, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control
See all VDAC1 / Porin primary antibodies -
Description
Rabbit polyclonal to VDAC1 / Porin - Mitochondrial Loading Control -
Host species
Rabbit -
Specificity
This antibody detects VDAC1, VDAC2 and VDAC3. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseChickenHuman
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Immunogen
Synthetic peptide corresponding to Human VDAC1/ Porin aa 150-250. The immunogen sequence is completely conserved between VDAC1, VDAC2 and VDAC3
(Peptide available asab16131) -
Positive control
- This antibody gave a positive control in the following human whole cell lysates: HeLa A431 Jurkat HEK293 This antibody gave a positive control in HeLa (Human epithelial carcinoma cell line) Nuclear This antibody gave a positive control in the following mouse tissue lysates: Heart Kidney Skeletal Muscle Spinal Cord This antibody gave a positive control in the following rat lysates: PC12 Whole Cell Brain Tissue Lysate - normal tissue Kidney Whole Cell Lysate - normal tissue
Images
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Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Hela Nuclear lysate
Lane 2 : Hela cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : Hela Nuclear lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 7 : Hela cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 8 : A431 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 9 : Jurkat cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 10 : HEK293 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)ab15895 stained in HeLa cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15895 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.
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Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Chicken liver cell lysate
Lane 2 : CHO K1 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : Chicken liver cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 5 : CHO K1 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lane 6 : MDCK cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
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Western blot - Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895)Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
20ug of Lysate per lane and detection using ab15895 diluted to 1ug/ml.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: HEK293 cell lysate
Lane 5: HepG2 cell lysate.
