Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5812] to TLS/FUS - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free
    See all TLS/FUS primary antibodies

  • Description

    Rabbit monoclonal [EPR5812] to TLS/FUS - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T, K562, HepG2 and HAP1 cell lysates. ICC/IF: K562 cells. IHC-P: Human kidney tissue.

  • General notes

    Ab232065 is the carrier-free version of ab124923. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232065 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    Western blot - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    All lanes : Anti-TLS/FUS antibody [EPR5812] (ab124923) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : FUS knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab124923).

      Lanes 1- 2: Merged signal (red and green). Green - ab124923 observed at 75 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab124923 was shown to react with TLS/FUS in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266587 (knockout cell lysate ab257100) was used. Wild-type HEK-293T and FUS knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124923 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)

    Immunocytochemistry/ Immunofluorescence analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling TLS/FUS with ab124923 at 1/250 (8.3 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised 0.1% TritonX-100. ab150077, AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/mL) was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    Confocal image showing nuclear staining in K-562 cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124923).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)

    ab124923, at 1/50 dilution, staining TLS/FUS in formalin-fixed, paraffin-embedded Human kidney tissue, by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124923).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    Western blot - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    All lanes : Anti-TLS/FUS antibody [EPR5812] (ab124923) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : TLS/FUS knockout HAP1 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 53 kDa



    Lanes 1 to 4: Merged signal (red and green). Green - ab124923 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab124923 was shown to specifically react with TLS/FUS when TLS/FUS knockout samples were used. Wild-type and TLS/FUS knockout samples were subjected to SDS-PAGE. ab124923 and ab28245 (loading control to GAPDH) were both diluted at 1/1,000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124923).

  • OI-RD Scanning - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    OI-RD Scanning - Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124923).

  • Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)
    Anti-TLS/FUS antibody [EPR5812] - BSA and Azide free (ab232065)

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