Antibodies, Proteins, Kits and Reagents for Life Science

Anti-TLS/FUS antibody [BLR023E] (ab243880)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR023E] to TLS/FUS
Suitable for: ICC/IF, IHC-P, IP, WB
Reacts with: Human

Product name

Anti-TLS/FUS antibody [BLR023E]
See all TLS/FUS primary antibodies
Description

Rabbit monoclonal [BLR023E] to TLS/FUS
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human TLS/FUS aa 1-50. The exact sequence is proprietary. Gene ID 2521.
Database link: P35637
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR023E
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-TLS/FUS antibody [BLR023E] (ab243880)

Formaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling TLS/FUS using ab243880 at 1/250 dilution in ICC/IF analysis. A DyLight594-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] (ab243880)

Formalin-fixed, paraffin-embedded human breast tissue stained for TLS/FUS using ab243880 at 1/250 dilution in immunohistochemical analysis. A DyLight594-conjugated goat anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS antibody [BLR023E] (ab243880)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TLS/FUS using ab243880 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunoprecipitation - Anti-TLS/FUS antibody [BLR023E] (ab243880)

TLS/FUS was immunoprecipitated from 1.0 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab243880 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243880 at 1/1000 dilution.
Lane 1: ab243880 IP in HEK-293T whole cell lysate.

Lane 2: Control IgG IP in HEK-293T whole cell lysate.

Detection: Chemiluminescence with an exposure time of 3 minutes.
Western blot - Anti-TLS/FUS antibody [BLR023E] (ab243880)

Western blot analysis using ab243880 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: HEK-293T whole cell lysate (50 µg).
Lane3: Jurkat whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds
Anti-TLS/FUS antibody [BLR023E] (ab243880)

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