Anti-TLS/FUS antibody (ab23439)
Key features and details
- Rabbit polyclonal to TLS/FUS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TLS/FUS antibody
See all TLS/FUS primary antibodies -
Description
Rabbit polyclonal to TLS/FUS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Non human primates
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TLS/FUS.
Read Abcam's proprietary immunogen policy (Peptide available as ab31610.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab23439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). Target
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Function
Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3.
Note=A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG.
Defects in FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein.
Defects in FUS are the cause of amyotrophic lateral sclerosis type 6 (ALS6) [MIM:608030]. ALS6 is a familial form of amyotrophic lateral sclerosis. ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10%. -
Sequence similarities
Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsArg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2521 Human
- Entrez Gene: 233908 Mouse
- Entrez Gene: 317385 Rat
- Omim: 137070 Human
- SwissProt: P35637 Human
- SwissProt: P56959 Mouse
- Unigene: 46894 Human
- Unigene: 277680 Mouse
see all -
Alternative names
- 75 kDa DNA pairing protein antibody
- 75 kDa DNA-pairing protein antibody
- ALS6 antibody
see all
Images
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Western blot - Anti-TLS/FUS antibody (ab23439)All lanes : Anti-TLS/FUS antibody (ab23439) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab23439 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Western blot - Anti-TLS/FUS antibody (ab23439)This image is courtesy of an anonymous AbreviewAnti-TLS/FUS antibody (ab23439) at 1/1000 dilution + Human 293T cell - whole cell lysate at 30000 cells
Secondary
An HRP Goat polyclonal to Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Blocking Step: 5% Milk; 1% BSA for 16 hours at 4°C
Datasheets and documents
References (21)
ab23439 has been referenced in 21 publications.
- Bian S miR-4319 inhibited the development of thyroid cancer by modulating FUS-stabilized SMURF1. J Cell Biochem 121:174-182 (2020). PubMed: 31148243
- Liu C et al. lncRNA RMST Suppressed GBM Cell Mitophagy through Enhancing FUS SUMOylation. Mol Ther Nucleic Acids 19:1198-1208 (2020). PubMed: 32069702
- Di W et al. Long noncoding RNA SNHG14 facilitates colorectal cancer metastasis through targeting EZH2-regulated EPHA7. Cell Death Dis 10:514 (2019). PubMed: 31273190
- Gasperini L et al. The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization. Mol Biol Cell N/A:mbcE18020108 (2018). PubMed: 30354839
- Liu C et al. A cytoplasmic long noncoding RNA LINC00470 as a new AKT activator to mediate glioblastoma cell autophagy. J Hematol Oncol 11:77 (2018). WB, IP ; Human . PubMed: 29866190
Images
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Western blot - Anti-TLS/FUS antibody (ab23439)All lanes : Anti-TLS/FUS antibody (ab23439) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab23439 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Western blot - Anti-TLS/FUS antibody (ab23439) This image is courtesy of an anonymous AbreviewAnti-TLS/FUS antibody (ab23439) at 1/1000 dilution + Human 293T cell - whole cell lysate at 30000 cells
Secondary
An HRP Goat polyclonal to Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding), 50 kDa (possible non-specific binding)
Blocking Step: 5% Milk; 1% BSA for 16 hours at 4°C
