Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)
![Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)](https://www.abcam.com/ps/products/255/ab255786/Images/ab255786-397822-anti-none-antibody-9g6-western-blot-wildtype-hap1-whole-cell-lysate.jpg "Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [9G6] to TLS/FUS (methyl ) - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free
See all TLS/FUS primary antibodies -
Description
Rat monoclonal [9G6] to TLS/FUS (methyl ) - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Other Immunogen Type. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1, K562 and NIH/3T3 whole cell lysate. Mouse brain cortex lysates. IHC-P: Human breast tissue. Mouse and rat cerebrum tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255786 is the carrier-free version of ab252830. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255786 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
9G6 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)All lanes : Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830) at 1/500 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TLS/FUS knockout HAP1 whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution
Predicted band size: 53 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?This data was developed using ab252830, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: lanes 1-3: 10 seconds; lane 4: 37 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)](https://www.abcam.com/ps/products/255/ab255786/Images/ab255786-3-anti-none-antibody-9g6-immunohistochemistry-human-breast.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)This data was developed using ab252830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human breast tissue labeling TLS/FUS with ab252830 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on human breast. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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![Western blot - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)](https://www.abcam.com/ps/products/255/ab255786/Images/ab255786-397823-anti-none-antibody-9g6-western-blot-mouse-brain-cortex-tissue-lysate-40-ug.jpg)
Western blot - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)Anti-TLS/FUS (methyl ) antibody [9G6] (ab252830) at 1/500 dilution + Mouse brain cortex tissue lysate at 40 µg
Secondary
Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10000 dilution
Predicted band size: 53 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?This data was developed using ab252830, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 46 seconds.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)](https://www.abcam.com/ps/products/255/ab255786/Images/ab255786-4-anti-none-antibody-9g6-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)This data was developed using ab252830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TLS/FUS with ab252830 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on mouse cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)](https://www.abcam.com/ps/products/255/ab255786/Images/ab255786-5-anti-none-antibody-9g6-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)This data was developed using ab252830, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TLS/FUS with ab252830 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
-
![Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)](https://www.abcam.com/ps/products/255/ab255786/Images/ab255786-6-benefits-of-recombinant-antibodies.png)
Anti-TLS/FUS (methyl ) antibody [9G6] - BSA and Azide free (ab255786)