Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3626] to ILF3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ILF3 antibody [EPR3626] - BSA and Azide free
    See all ILF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR3626] to ILF3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    ab232546 is the carrier-free version of ab92355.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)
    Flow Cytometry - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ILF3 with purified ab92355 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92355).

  • Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)
    Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)

    Immunofluorescence staining of HeLa cells with purified ab92355 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92355).

  • Flow Cytometry - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)
    Flow Cytometry - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)

    Overlay histogram showing HeLa cells stained with unpurified ab92355 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92355, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92355).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)

    Immunohistochemical analysis of paraffin embedded human kidney tissue stained for ILF3 using ab92355 (unpurified) at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92355).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)
    Anti-ILF3 antibody [EPR3626] - BSA and Azide free (ab232546)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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