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Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14123(B)] to CRM1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free
    See all CRM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14123(B)] to CRM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250153 is the carrier-free version of ab180144.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    Western blot - Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    All lanes : Anti-CRM1 antibody [EPR14123(B)] (ab180144) at 1/2000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 123 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180144, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    Immunocytochemistry - Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    This data was developed using ab180144, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling CRM1 with Purified ab180144 at 1/500 dilution. Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
  • Flow Cytometry - Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    Flow Cytometry - Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    This data was developed using ab180144, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CRM1 with ab180144 at 1/200. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG.
  • Immunocytochemistry - Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    Immunocytochemistry - Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    This data was developed using ab180144, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 20°C acetone fixed K562 cells labeling CRM1 with ab180144 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(Dylight 488) .
  • Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)
    Anti-CRM1 antibody [EPR14123(B)] - BSA and Azide free (ab250153)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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