HRP Anti-ILF3 antibody [EPR3626] (ab206250)
![HRP Anti-ILF3 antibody [EPR3626] (ab206250)](https://www.abcam.com/ps/products/206/ab206250/Images/ab206250-414405-AntiILF3-antibody.jpg "HRP Anti-ILF3 antibody [EPR3626] (ab206250)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR3626] to ILF3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-ILF3 antibody [EPR3626]
See all ILF3 primary antibodies -
Description
HRP Rabbit monoclonal [EPR3626] to ILF3 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji, HeLa and K562 whole cell lysates. IHC-P: normal human kidney tissue sections
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General notes
This product was previously labelled as ILF3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3626 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-ILF3 antibody [EPR3626] (ab206250)All lanes : HRP Anti-ILF3 antibody [EPR3626] (ab206250) at 1/5000 dilution
Lane 1 : Raji Whole Cell lysate
Lane 2 : HeLa Whole Cell lysate
Lane 3 : K562 Whole Cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 95 kDaBlocking buffer: 3% Milk
Gel type: MOPS
Observed band size: 95 kDa
Exposure Time: 2 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (ab206250)](https://www.abcam.com/ps/products/206/ab206250/Images/ab206250-267178-IHCAP00237271.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (ab206250)IHC image of ILF3 staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab206250, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![HRP Anti-ILF3 antibody [EPR3626] (ab206250)](https://www.abcam.com/ps/products/206/ab206250/Images/ab206250-5-benefits-of-recombinant-antibodies.png)
HRP Anti-ILF3 antibody [EPR3626] (ab206250)
