Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13087(B)] to PPA2 - BSA and Azide free
  • Suitable for: IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free
    See all PPA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13087(B)] to PPA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250010 is the carrier-free version of ab177935 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250010 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)
    Western blot - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)
    All lanes : Anti-PPA2 antibody [EPR13087(B)] (ab177935) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Human fetal kidney tissue lysate
    Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 5 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 38 kDa



    This data was developed using ab177935, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)
    Flow Cytometry - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)
    This data was developed using ab177935, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized 293 cells labeling PPA2 with ab177935 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
  • Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)
    Immunoprecipitation - Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)
    This data was developed using ab177935, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from U87-MG cell lysate using ab177935.
  • Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)
    Anti-PPA2 antibody [EPR13087(B)] - BSA and Azide free (ab250010)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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