Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ILF3 antibody [EPR3626] (ab92355)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3626] to ILF3
  • Suitable for: WB, IHC-P, Flow Cyt, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ILF3 antibody [EPR3626]
    See all ILF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR3626] to ILF3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ILF3. The exact sequence is proprietary.

  • Positive control

    • Raji, HeLa and K562 cell lysates. Human kidney tissue.

  • General notes

     This product was previously labelled as ILF3

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ILF3 antibody [EPR3626] (ab92355)
    Western blot - Anti-ILF3 antibody [EPR3626] (ab92355)
    All lanes : Anti-ILF3 antibody [EPR3626] (ab92355) at 1/100000 dilution

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 95 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.
    The two bands are reported by PMID: 22842455 and 27872311.

  • Immunocytochemistry - Anti-ILF3 antibody [EPR3626] (ab92355)
    Immunocytochemistry - Anti-ILF3 antibody [EPR3626] (ab92355)

    Immunofluorescence staining of HeLa cells with purified ab92355 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Flow Cytometry - Anti-ILF3 antibody [EPR3626] (ab92355)
    Flow Cytometry - Anti-ILF3 antibody [EPR3626] (ab92355)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ILF3 with purified ab92355 at 1:60 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-ILF3 antibody [EPR3626] (ab92355)
    Western blot - Anti-ILF3 antibody [EPR3626] (ab92355)
    All lanes : Anti-ILF3 antibody [EPR3626] (ab92355) at 1/100000 dilution (unpurified)

    Lane 1 : Raji cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

    Predicted band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] (ab92355)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] (ab92355)

    ab92355 (unpurified) at 1/100 dilution staining ILF3 in paraffin-embedded Human kidney tissue, by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ILF3 antibody [EPR3626] (ab92355)
    Flow Cytometry - Anti-ILF3 antibody [EPR3626] (ab92355)

    Overlay histogram showing HeLa cells stained with unpurified ab92355 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92355, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-ILF3 antibody [EPR3626] (ab92355)
    Anti-ILF3 antibody [EPR3626] (ab92355)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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