Anti-ICAM1 antibody [MEM-111] (ab2213)
![Anti-ICAM1 antibody [MEM-111] (ab2213)](https://www.abcam.com/ps/products/2/ab2213/Images/ab2213-274618-anti-icam1-antibody-mem-111-western-blot.jpg "Anti-ICAM1 antibody [MEM-111] (ab2213)")
Key features and details
- Mouse monoclonal [MEM-111] to ICAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-ICAM1 antibody [MEM-111]
See all ICAM1 primary antibodies -
Description
Mouse monoclonal [MEM-111] to ICAM1 -
Host species
Mouse -
Specificity
his antibody reacts with human CD54 antigen.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Tissue/ cell preparation (Human). (Burkitt's lymphoma cell line RAJI).
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Positive control
- For WB it is highly recommended to use TNF-alpha activated HUVEC cells as a positive control.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-111 -
Myeloma
unknown -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Images
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Western blot - Anti-ICAM1 antibody [MEM-111] (ab2213)All lanes : Anti-ICAM1 antibody [MEM-111] (ab2213)
Lane 1 : TNF-alpha activated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : TNF-alpha nonactivated HUVEC cells
Performed under non-reducing conditions.
Observed band size: 90 kDa why is the actual band size different from the predicted?Lower bands represent tubulin as a loading control.
