Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ICAM1 antibody [EPR16608] (ab179707)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16608] to ICAM1
Suitable for: IHC-P, WB
Reacts with: Mouse

Product name

Anti-ICAM1 antibody [EPR16608]
See all ICAM1 primary antibodies
Description

Rabbit monoclonal [EPR16608] to ICAM1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse spleen, liver, kidney and lung lysates; bEnd.3, A20, RAW 264.7 and WEHI-231 whole cell lysates. IHC-P: Mouse liver and kidney tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16608
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (ab179707)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ICAM1 with ab179707 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Positive staining on endothelial cells of mouse liver is observed [PMID: 17058232].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM1 antibody [EPR16608] (ab179707)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ICAM1 with ab179707 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Positive staining on endothelial cells of mouse kidney is observed [PMID: 17058232].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-ICAM1 antibody [EPR16608] (ab179707)

All lanes : Anti-ICAM1 antibody [EPR16608] (ab179707) at 1/1000 dilution

Lane 1 : Mouse spleen lysate at 20 µg
Lane 2 : Mouse liver lysate at 20 µg
Lane 3 : Mouse kidney lysate at 20 µg
Lane 4 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate at 10 µg
Lane 5 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 58 kDa
Observed band size: 80-110 kDa
why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The observed molecular weight is consistent with the literature (PMID: 1712683).
Western blot - Anti-ICAM1 antibody [EPR16608] (ab179707)

All lanes : Anti-ICAM1 antibody [EPR16608] (ab179707) at 1/1000 dilution

Lane 1 : Mouse lung lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 58 kDa
Observed band size: 80-110 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/2: 1 second; Lane 3: 10 seconds.

The observed molecular weight is consistent with the literature (PMID: 1712683).
Anti-ICAM1 antibody [EPR16608] (ab179707)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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