Anti-FRYL antibody (ab95065)
Key features and details
- Rabbit polyclonal to FRYL
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-FRYL antibody
See all FRYL primary antibodies -
Description
Rabbit polyclonal to FRYL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Synthetic peptide, corresponding to a region within amino acids 2600-2650 of Human FRYL (NP_055845.1).
-
Positive control
- HeLa whole cell lysate (ab150035), 293T cell lysate, Mouse NIH3T3 cell lysate,
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
-
Western blot - Anti-FRYL antibody (ab95065)All lanes : Anti-FRYL antibody (ab95065) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 340 kDa
Exposure time: 10 seconds
-
Immunoprecipitation - Anti-FRYL antibody (ab95065)Lane 1: Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab95065 at 1 µg/ml.
Developed using the ECL technique with an exposure time of 1 seconds.
