Astana Biomed Group, an authorized Abcam distributor in Central Asia

HRP Anti-Rad51 antibody [EPR4030(3)] (ab195548)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • HRP Rabbit monoclonal [EPR4030(3)] to Rad51
  • Suitable for: IHC-P
  • Reacts with: Human
  • Conjugation: HRP

Overview

  • Product name

    HRP Anti-Rad51 antibody [EPR4030(3)]
    See all Rad51 primary antibodies

  • Description

    HRP Rabbit monoclonal [EPR4030(3)] to Rad51

  • Host species

    Rabbit

  • Conjugation

    HRP

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: normal human testis tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Dissociation constant (KD)

    KD = 9.20 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.40
    Preservative: 0.1% 10% Proclin 300 Solution
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR4030(3)

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rad51 antibody [EPR4030(3)] (ab195548)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Rad51 antibody [EPR4030(3)] (ab195548)

    IHC image of Rad51 staining in a section of formalin-fixed paraffin-embedded normal human testis tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195548 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • HRP Anti-Rad51 antibody [EPR4030(3)] (ab195548)
    HRP Anti-Rad51 antibody [EPR4030(3)] (ab195548)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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