Anti-POU5F1B antibody (ab230429)
Key features and details
- Rabbit polyclonal to POU5F1B
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-POU5F1B antibody -
Description
Rabbit polyclonal to POU5F1B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Pig
-
Immunogen
Synthetic peptide within Human POU5F1B aa 214-242 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q06416 -
Positive control
- WB: K562 cell lysate. IHC-P: Human testis tissue. Flow Cyt: K562 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-POU5F1B antibody (ab230429)Anti-POU5F1B antibody (ab230429) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 35 µg
Predicted band size: 39 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POU5F1B antibody (ab230429)Formalin-fixed, paraffin-embedded human testis tissue stained for POU5F1B using ab230429 at 1/100 dilution in immunohistochemical analysis.
-
Flow Cytometry - Anti-POU5F1B antibody (ab230429)Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling POUF5F1B with ab230429 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used.