Human THBS2 ELISA Kit (ab260051)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.024 ng/ml
- Range: 0.09 ng/ml - 6 ng/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Rhesus monkey
Overview
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Product name
Human THBS2 ELISA Kit
See all Thrombospondin 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.5% Inter-assay Sample n Mean SD CV% Serum 3 1.3% -
Sample type
Milk, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.024 ng/ml -
Range
0.09 ng/ml - 6 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Milk 123 121% - 125% Serum 120 112% - 129% Cell culture media 110 107% - 113% Hep Plasma 118 114% - 121% EDTA Plasma 114 105% - 123% Cit plasma 112 102% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human, Rhesus monkey
Does not react with: Cow -
Product overview
Human THBS2 ELISA Kit (ab260051) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of THBS2 protein in cit plasma, edta plasma, hep plasma, milk, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human THBS2 with 0.024 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
THBS2 (Thrombospondin-2) is a secreted cell adhesion glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. It is a ligand for CD36 which mediates antiangiogenic properties.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human THBS2 Capture Antibody 1 x 600µl 10X Human THBS2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human THBS2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 75BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. -
Tissue specificity
High expression in invertebral disk tissue. -
Involvement in disease
Genetic variations in THBS2 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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Alternative names
- THBS2
- Thrombospondin-2
- TSP2
- TSP2_HUMAN
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Database links
- Entrez Gene: 7058 Human
- Omim: 188061 Human
- SwissProt: P35442 Human
- Unigene: 371147 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human THBS2 standard curve comparisonStandard Curve comparison between human THBS2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human THBS2 standard curve in Sample Diluent 75BS.The THBS2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native THBS2 in human serum and plasma samples.The concentrations of THBS2 were measured in duplicates, interpolated from the THBS2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 50%, plasma (heparin) 25%, plasma (EDTA) 50%, and plasma (CTAD) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean THBS2 concentration was determined to be 17.55 ng/mL in neat serum, 8.328 ng/mL in neat plasma (citrate), 18.15 ng/mL in neat plasma (heparin), 11.22 ng/mL in neat plasma (EDTA), and 8.524 ng/mL in neat plasma (CTAD).
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. Interpolated concentrations of native THBS2 in human breast milk samples.The concentrations of THBS2 were measured in duplicates, interpolated from the THBS2 standard curves and corrected for sample dilution. Undiluted samples are as follows: breast milk 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean THBS2 concentration was determined to be 0.825 ng/mL in neat human breast milk (defatted).
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Serum from nine individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean THBS2 concentration was determined to be 17.83 ng/mL with a range of 12.01 – 21.94 ng/mL.
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Sandwich ELISA - Human THBS2 ELISA Kit (ab260051)To learn more about the advantages of recombinant antibodies see here.
