Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14110(2)] to RAB13 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free
    See all RAB13 primary antibodies

  • Description

    Rabbit monoclonal [EPR14110(2)] to RAB13 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HEK-293, Caco-2, A431 and HeLa whole cell lysates; human fetal lung lysate.

  • General notes

    Ab251431 is the carrier-free version of ab205528. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251431 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    Western blot - Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    All lanes : Anti-RAB13 antibody [EPR14110(2)] (ab205528) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : RAB13 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : HAP1 whole cell lyate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab205528, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab205528 observed at 24 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab205528 Anti-RAB13 antibody [EPR14110(2)] was shown to specifically react with RAB13 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266843 (knockout cell lysate ab258159) was used. Wild-type and RAB13 knockout samples were subjected to SDS-PAGE. ab205528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    Western blot - Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    All lanes : Anti-RAB13 antibody [EPR14110(2)] (ab205528) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : RAB13 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 23 kDa



    This data was developed using ab205528, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab205528 observed at 23 kDa. Red - loading control, ab9484, observed at 37 kDa.

     

    ab205528 was shown to specifically react with RAB13 in wild-type HAP1 cells as signal was lost in RAB13 knockout cells. Wild-type and RAB13 knockout samples were subjected to SDS-PAGE. ab205528 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    Western blot - Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    All lanes : Anti-RAB13 antibody [EPR14110(2)] (ab205528) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 1 minute


    This data was developed using ab205528, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    Western blot - Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    Anti-RAB13 antibody [EPR14110(2)] (ab205528) at 1/2000 dilution + Human fetal lung lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 3 minutes


    This data was developed using ab205528, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)
    Anti-RAB13 antibody [EPR14110(2)] - BSA and Azide free (ab251431)

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