Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP442Y] to ARF1 + ARF3
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-ARF1 + ARF3 antibody [EP442Y]
    See all ARF1+ARF3 primary antibodies

  • Description

    Rabbit monoclonal [EP442Y] to ARF1 + ARF3

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 100% homology with ARF3, ARF4 and ARF5. Cross-reactivity with the latter two protein have not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ARF1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HEK-293 cell lysates and human breast carcinoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    All lanes : Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524) at 1/20000 dilution

    Lane 1 : Full length human ARF-1 recombinant protein
    Lane 2 : Full length human ARF-3 recombinant protein

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

    Human ARF-1 recombinant protein, (Cat#:ab123538 )
    Human ARF-3 recombinant protein, (Cat#:ab113581 )

     

  • Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    Immunocytochemistry/ Immunofluorescence - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)

    Immunofluorescence staining of MCF7 cells with purified ab32524 working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

  • Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    Western blot - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524) at 1/2000 dilution + HEK-293 cell lysate

    Predicted band size: 21 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab32524 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    Flow Cytometry - Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    Overlay histogram showing HeLa cells stained with ab32524 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32524, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)
    Anti-ARF1 + ARF3 antibody [EP442Y] (ab32524)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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