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Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)

Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [100/D5] to Bcl-2 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Bcl-2 antibody [100/D5] - BSA and Azide free
    See all Bcl-2 primary antibodies
  • Description

    Mouse monoclonal [100/D5] to Bcl-2 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: CowDoes not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Bcl-2 aa 1-100 (Cysteine residue).
    Database link: P10415

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Jurkat, K562, HL60, or HeLa Cells. Tonsil or follicular lymphomas. Non- Hodgkin's lymphoma tissue. Human skin lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab212800 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    100/D5
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Cell survival & death
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Images

  • Western blot - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
    Western blot - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
    Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800) at 1 µg/ml + Human skin lysate

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human non-Hodgkin's Lymphoma tissue labeling Bcl-2 with ab212800 at 1 μg/ml.

  • Flow Cytometry - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)
    Flow Cytometry - Anti-Bcl-2 antibody [100/D5] - BSA and Azide free (ab212800)

    Flow cytometric analysis of Jurkat cells labeling Bcl-2 uwith ab212800 at 1µg/106 cells (red), compared with an isotype control (green).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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