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Anti-Aspartate Aminotransferase antibody [C1] (ab239487)

Key features and details

  • Mouse monoclonal [C1] to Aspartate Aminotransferase
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Aspartate Aminotransferase antibody [C1]
    See all Aspartate Aminotransferase primary antibodies

  • Description

    Mouse monoclonal [C1] to Aspartate Aminotransferase

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    Pig
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human Aspartate Aminotransferase aa 1-450. N-terminal His Tag. Expressed in E.coli.
    Database link: P17174

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Recombinant Aspartate Aminotransferase protein; Human and mouse liver lysates; Rat liver, brain and heart lysate; Pig brain and heart lysate. HAP1, HeLa and Raw264.7 cell lysates. IHC-P: Human kidney tissue.

Properties

Images

  • Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    All lanes : Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 0.5 µg/ml

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : GOT1 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Raw264.7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.


    Lanes 1 - 4:  Merged signal (red and green). Green - ab239487 observed at 46 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

    ab239487 was shown to react with Aspartate Aminotransferase in wild-type HAP1 cells in western blot. Loss of signal was observed when GOT1 knockout sample was used. Wild-type and GOT1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab239487 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    All lanes : Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 2 µg/ml

    Lane 1 : Pig brain lysate
    Lane 2 : Pig heart lysate

    Secondary
    All lanes : HRP-linked Rabbit anti-Mouse IgG at 1/5000 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)

    Paraffin-embedded human kidney tissue stained for Aspartate Aminotransferase using ab239487 at 20 μg/ml in immunohistochemical analysis. DAB staining.

  • Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Rat heart lysate

    Secondary
    HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml
  • Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Rat brain lysate

    Secondary
    HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml
  • Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Rat liver lysate

    Secondary
    HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml
  • Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Mouse liver lysate

    Secondary
    HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml
  • Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 3 µg/ml + Human liver lysate

    Secondary
    HRP-linked caprine anti-mouse polyclonal IgG at 0.2 µg/ml
  • Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Western blot - Anti-Aspartate Aminotransferase antibody [C1] (ab239487)
    Anti-Aspartate Aminotransferase antibody [C1] (ab239487) at 5 µg/ml + Recombinant human aspartate aminotransferase protein

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