Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase + FABP-1 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase + FABP-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human glioma tissue.

  • General notes

    Ab232471 is the carrier-free version of ab170950. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232471 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950) at 1/1000 dilution + Recombinant human FABP-1 protein (ab206788) at 0.015 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).
  • Flow Cytometry - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Flow Cytometry - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

    Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1:20 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/120. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue). 

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

    Immunofluorescent analysis of HepG2 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution (green). DAPI nuclear staining (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Flow Cytometry - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Flow Cytometry - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

    Flow cytometric analysis of permeabilized K562 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/10 dilution (red)  or a rabbit IgG negative (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling Aspartate Aminotransferase + FABP-1 with Purified ab170950 at 1:170 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)
    Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (ab232471)

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