Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24384-69] to Carboxypeptidase A - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free
    See all Carboxypeptidase A primary antibodies

  • Description

    Rabbit monoclonal [EPR24384-69] to Carboxypeptidase A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human pancreas tisue lysate; Mouse pancreas tissue lysate; Rat pancreas tissue lysate. IHC-P: Human pancreas tissue; Mouse pancreas tissue; Rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; Rat pancreas tissue.

  • General notes

    ab278044 is the carrier-free version of ab278047. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278044 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

    This data was developed using ab278044, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on acinar cells of human pancreas (PMID: 23006325). The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

    This data was developed using ab278044, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/100 (5.39 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse pancreas is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    All lanes : Anti-Carboxypeptidase A antibody [EPR24384-69] (ab278044) at 1/1000 dilution

    Lane 1 : Human pancreas tissue lysate
    Lane 2 : Mouse pancreas tissue lysate
    Lane 3 : Rat pancreas tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 33,43 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab278044, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 21098023).

    Activation of pro-carboxypeptidase A is initiated by proteolytic cleavage at the C-terminal end of the propeptide by trypsin and chymotrypsin (PMID: 8436102, 21098023).

    Exposure time: 3 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

    This data was developed using ab278044, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on acinar cells of mouse pancreas. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

    This data was developed using ab278044, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/500 (1.078 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) at 1/1000 dilution (Green). Positive staining on rat pancreas is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

    This data was developed using ab278044, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on acinar cells of rat pancreas. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

    This data was developed using ab278044, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Carboxypeptidase A with ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control: No staining on human spleen. The section was incubated with ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
    Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

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