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Microbiology Interspecies Interaction Host Virus Interaction

Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)

Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16288] to UQCRFS1/RISP - BSA and Azide free
  • Suitable for: ICC, IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free
    See all UQCRFS1/RISP primary antibodies
  • Description

    Rabbit monoclonal [EPR16288] to UQCRFS1/RISP - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human UQCRFS1/RISP aa 50-150. The exact sequence is proprietary.
    Database link: Q69BK6

  • General notes

    Ab251053 is the carrier-free version of ab191078. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251053 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR16288
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Cell Biology
    • Other Antibodies
    • Plant related targets
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex III

Images

  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Raji lysates
    Lane 3 : 293 lysates
    Lane 4 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab191078, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Western blot - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    All lanes : Anti-UQCRFS1/RISP antibody [EPR16288] (ab191078) at 1/1000 dilution

    Lane 1 : mouse brain lysate
    Lane 2 : mouse heart lysate
    Lane 5 : rat heart lysate
    Lane 6 : rat kidney lysate
    Lane 7 : RAW264.7 lysate
    Lane 8 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?



    This data was developed using ab191078, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunoprecipitation - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)

    This data was developed using ab191078, the same antibody clone in a different buffer formulation.

    Western blot analysis of UQCRFS1/RISP in immunoprecipitation pellets from Human fetal lysate. ab191078 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.

  • Immunocytochemistry - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunocytochemistry - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    This data was developed using ab191078, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of methanol-fixed HeLa cells labeling UQCRFS1/RISP with ab191078 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). Negative controls also shown.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    This data was developed using ab191078, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    This data was developed using ab191078, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    This data was developed using ab191078, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling UQCRFS1/RISP with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)
    Anti-UQCRFS1/RISP antibody [EPR16288] - BSA and Azide free (ab251053)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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